目的:建立HPLC同时测定铁包金不同部位中大黄素、大黄酚、大黄素甲醚的含量。方法:色谱柱为迪马ODS C18(250 mm×4.6 mm,5μm);流动相为甲醇-0.2%磷酸溶液(74∶26);流速:1.0 mL/min;柱温:35℃;检测波长:254 nm。结果:大黄素、大黄酚、大黄素甲醚分别在进样量为0.00201~0.0804μg(r=0.9998),0.0066~0.264μg(r=0.9997),0.0124~0.496(r=0.9998)范围内与峰面积线性关系良好;平均加样回收率分别为100.43%、101.29%、98.36%。铁包金根的总蒽醌含量高于藤茎。结论:该方法简便、重复性好,结果准确可靠,可用于铁包金药材的质量控制。 OBJECTIVE: To establish an HPLC method for the simultaneous determination of emodin, chrysophanol and physcion in different parts of the ferrules. METHODS: The column was Dima ODS C18 (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol-0.2% phosphoric acid solution (74:26), the flow rate was 1.0 mL / min, the column temperature was 35 ℃, 254 nm. RESULTS: Emodin, chrysophanol and physcion were separated from peaks in the range of 0.00201 ~ 0.0804μg (r = 0.9998), 0.0066 ~ 0.264μg (r = 0.9997) and 0.0124 ~ 0.496 The linear relationship was good. The average recoveries were 100.43%, 101.29% and 98.36% respectively. The total anthraquinone content of iron-coated roots is higher than that of cane. Conclusion: The method is simple and reproducible, the results are accurate and reliable, and can be used for quality control of iron-clad gold medicinal materials.