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应用全细胞膜片钳记录技术在大鼠新鲜分离DRG神经元上,观察预加多巴胺D_2受体选择性激动剂R(—)—NPA对GABA—激活电流的调制作用.绝大部分受检细胞86.2%(50/58)对外加GABA敏感.10~(-6)～10~(-3)mol/LGABA 引起一剂量依赖性有明显去敏感作用的内向电流.对GABA敏感的50个受检细胞中13个细胞引起一较大的无明显去敏感的内向电流,其他的无反应.预加R(一)—NPA30秒后再加GABA,则对GABA—激活电流幅值的影响如下:抑制的为76%(38/5O),增强的为2%(1/50),无明显作用的为22%(11/5O).在其浓度为10~(-4)mol/L、10~(-5)mol/L、10~(-6)mol/L、10~(-7)moV/L时抑制(X±SE)分别为19.1%12.8(n=6)、42.49%±3.2(n=6)、83.6%±1.2(n=6)、8.69%±1.8(n=6).细胞内加蛋白激酶抑制剂H7后,R(—)—NPA对GABA的抑制作用完全消除(n=16).
Whole-cell patch-clamp recording technique was used to investigate the modulation of GABA-activated current by R (-) - NPA, a selective agonist of dopamine D 2 receptor, in freshly isolated DRG neurons of rats.Most of the cells tested 86.2 % (50/58) was sensitive to GABA plus 10 ~ (-6) ~ 10 ~ (-3) mol / LGABA induced a desensitized inward current in a dose-dependent manner.50 of GABA-sensitive cells 13 of the cells caused a large inrush current without significant desensitization and no other reaction.After 30 seconds of pretreatment with R (a) -NPA plus GABA, the effect on GABA-activated current amplitude was as follows: Was 76% (38/50), enhanced by 2% (1/50) and no significant effect was 22% (11/50) (X ± SE) were 19.1% 12.8 (n = 6) and 42.49% ± 3.2 (n = 6) when the concentration was 10 -5 mol / L, 10 -6 mol / L and 10 -7 mol / = 6), 83.6% ± 1.2 (n = 6), 8.69% ± 1.8 (n = 6) .After intracellular addition of the protein kinase inhibitor H7, the inhibitory effect of R (-) - NPA on GABA was completely eliminated (n = 16).