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目的观察急性心肌梗死(acute myocardial infarction,AMI)患者骨髓单核细胞移植手术前后血浆尾加压素Ⅱ(UⅡ)和内皮素-1(ET-1)水平的变化,探讨骨髓单核细胞移植对血管活性物质的影响。方法采集23例AMI患者在骨髓单核细胞移植术前、术后1 d及术后7 d静脉血,应用酶联免疫法测定血浆UⅡ和ET-1水平。结果正常对照组人群血浆UⅡ浓度为(0.462±0.115)mg/L,AMI患者血浆UⅡ浓度(0.223±0.043)mg/L较对照组明显低下(P<0.05)。骨髓单核细胞移植1 d后,血浆UⅡ浓度有所升高,为移植术前的129%(P<0.05)。移植7 d后,血浆UⅡ浓度回降到移植前的114%,但与移植后1 d比较差异无统计学意义。与骨髓单核细胞移植术前比较,血浆ET-1水平在移植后1 d明显降低,为移植前的61%(P<0.01),移植7 d后,ET-1水平较移植后1 d略有回升,为移植前的71%(P<0.01)。结论血浆UⅡ和ET-1的水平在骨髓单核细胞选择性移植术前后发生明显改变,为探讨骨髓单核细胞移植改善心脏功能的机制提供了理论依据。
Objective To investigate the changes of urotensin Ⅱ (UⅡ) and endothelin-1 (ET-1) levels in patients with acute myocardial infarction (AMI) before and after bone marrow mononuclear cells transplantation. To investigate the effect of bone marrow mononuclear cell transplantation Effects of Vasoactive Substances. Methods Twenty-three patients with acute myocardial infarction (AMI) were enrolled in this study. The levels of plasma UⅡ and ET-1 were measured by enzyme-linked immunosorbent assay before transplantation, 1 d after operation and 7 d after operation. Results The plasma concentration of UⅡ was (0.462 ± 0.115) mg / L in normal control group and lower than that in control group (0.223 ± 0.043) mg / L in AMI group (P <0.05). One day after transplantation of bone marrow mononuclear cells, the concentration of plasma UⅡ increased to 129% before transplantation (P <0.05). After 7 days of transplantation, the plasma UⅡ concentration dropped back to 114% before transplantation, but there was no significant difference compared with that on day 1 after transplantation. Compared with the pre-transplantation bone marrow mononuclear cells, the plasma ET-1 level significantly decreased on the 1st day after transplantation, which was 61% (P <0.01) before transplantation. After 7 days of transplantation, the ET-1 level was slightly decreased There was a rise of 71% before transplantation (P <0.01). Conclusions The levels of plasma UⅡ and ET-1 are significantly changed before and after selective bone marrow mononuclear cells transplantation, which provides a theoretical basis for exploring the mechanism of cardiac myeloid leukemia transplantation.