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目的:通过实验研究不同剂量地塞米松对骨代谢的影响。方法:把36只三个月龄的雌性鼠随机分为A、B、C、D四组,每组8只。A组8只大鼠作为对照组,B、C、D三组分别给予1mg/kg、2.5mg/kg、、5mg/kg三种不同浓度的地塞米松尾静脉注射。并对四组大鼠进行骨生物指标测定,以及检验细胞周期、Caspase3活性。结果:通过比较A、B、C、D四组处理结果发现,大鼠骨矿含量与地塞米松的浓度呈负相关,其随着地塞米松的浓度的增加而降低;相比A、C、D三组,低剂量的B组8只大鼠的最大压缩载荷明显较少(P值小于0.05);注射不同剂量地塞米松的B、C、D三组大鼠成骨细胞的凋亡率以及Caspase3活性相对A组明显增加(P值小于0.05),而且低剂量的B组凋亡率以及Caspase3活性与C、D两组相比较少(P值小于0.05)。结论:应用地塞米松诱导细胞凋亡,降低骨生物学质量,其影响程度随剂量的变化而变化,且激活Caspase-3活性是地塞米松引起成骨细胞凋亡的重要途径。
OBJECTIVE: To study the effects of different doses of dexamethasone on bone metabolism through experiments. Methods: 36 three-month-old female rats were randomly divided into four groups A, B, C and D, with 8 in each group. A group of 8 rats as a control group, B, C, D three groups were given 1mg / kg, 2.5mg / kg ,, 5mg / kg three different concentrations of dexamethasone tail vein injection. The bone biomarkers were measured in four groups of rats, and the cell cycle and Caspase3 activity were also tested. Results: By comparing the results of four groups A, B, C and D, it was found that there was a negative correlation between bone mineral content and the concentration of dexamethasone in rats, which decreased with the concentration of dexamethasone. D group, 8 rats in low dose group B had significantly lower maximum compressive load (P value less than 0.05). The apoptosis rates of osteoblasts in B, C and D groups with different doses of dexamethasone (P <0.05), and the apoptotic rate and the activity of Caspase3 in low dose group B were less than those in group C and D (P <0.05). CONCLUSION: Dexamethasone can induce apoptosis and decrease the quality of bone biology. The effect of dexamethasone is dose dependent. Activation of Caspase-3 activity is an important pathway of dexamethasone-induced apoptosis of osteoblasts.