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A description of a successful direct somatic embryogenesis induction from immature zygotic embryos of a camphor tree (Cinnamomum camphora L.) is presented. After a subculture of 2–3 years, embryogenic calli could be derived from primary somatic embryos. Immature zygotic embryos were cultured on a Murashige and Skoog (MS) basal medium supplemented with a range of combinations of cytokinins (BA) and auxins (2,4-D or NAA) for somatic embryo induction. Primary somatic embryos could be in-duced directly in almost all PGR combinations. A positive effect of 2,4-D on somatic embryogenesis from immature zygotic embryos of camphor tree was obtained. BA at appropriate concentrations (< 5 mg·L–1) had an effect similar to 2,4-D, whereas high concentra-tions (>5 mg·L–1) of BA had the effect of restraining somatic embryo induction. NAA had a less positive effect on somatic embryo-genesis than 2,4-D.
A description of a successful direct somatic embryogenesis induction from immature zygotic embryos of a camphor tree (Cinnamomum camphora L.) is presented. After a subculture of 2-3 years, embryogenic calli could be derived from primary somatic embryos. Immature zygotic embryos were cultured on a Murashige and Skoog (MS) basal medium supplemented with a range of combinations of cytokinins (BA) and auxins (2,4-D or NAA) for somatic embryo induction. Primary somatic embryos could be in-duced directly in almost all PGR A positive effect of 2,4-D on somatic embryogenesis from immature zygotic embryos of camphor tree was obtained. BA at appropriate concentrations (<5 mg · L -1) had an effect similar to 2,4-D, while high concentra-tions (> 5 mg · L-1) of BA had the effect of restraining somatic embryo induction. NAA had a less positive effect on somatic embryo-genesis than 2,4-D.