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以添加镇静催眠候选新药N6-羟苄腺嘌呤核苷[N6-(4-hydroxybenzyl)adenine riboside,NHBA]的组织匀浆切片作为考察对象,对空气动力辅助离子化质谱成像系统(Air Flow Assisted Ionization Mass Spectrometry Imaging,AFAI-MSI)的关键参数进行考察与优化,以保证最佳条件进行样品检测。在此基础上,对预处理的整体大鼠组织切片进行平行的连续两次质谱成像分析,考察了其内源性代谢物在成像分析过程中是否发生变化。通过对采集数据中关键质谱峰的筛选等处理步骤,并采用可视化的主成分分析(PCA)方法,开展了组织样品内源性代谢物的稳定性分析,最终验证了采用该样品前处理和质谱成像方法,能够保证组织切片样品中内源性代谢物的稳定性,为质谱成像分析结果提供了可靠依据。
The tissue homogenate slice of N6- (4-hydroxybenzyl) adenine riboside (NHBA), a new sedative and hypnotic candidate drug, was added to investigate the effects of Air Flow Assisted Ionization Mass Spectrometry Imaging, AFAI-MSI) to investigate and optimize the key parameters to ensure the best conditions for sample testing. On this basis, the parallel preconditioned whole rat tissue sections were subjected to two consecutive mass spectrometric imaging analyzes to examine whether endogenous metabolites changed during the imaging analysis. The stability analysis of the endogenous metabolites of the tissue samples was carried out by using the principal components analysis (PCA) method of visualization and other processing steps such as screening of the key mass peaks in the collected data. Finally, the sample preparation and the mass spectrometry Imaging method, to ensure the stability of endogenous metabolites in the tissue section samples provide a reliable basis for mass spectrometry imaging analysis results.