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[目的]探讨多胺引发对黑麦草胚生理生化和种子发芽的影响。[方法]以一年生黑麦草(钻石T和牧乐8000)为材料,种子经0.5mmol/L的腐胺(Put)、亚精胺(Spd)和精胺(Spm)引发24h后,在5℃下低温吸胀不同时间(12、24、36和48h),分析Put、Spd和Spm引发对一年生黑麦草种子吸胀中耐冷性和种子发芽能力的影响。[结果]Put、Spd和Spm引发提高了胁迫期间种子胚内过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和抗坏血酸过氧化物酶(APX)活性,以及可溶性蛋白质含量,同时也显著提高发芽率、缩短平均发芽时间。低温吸胀48h后,与对照相比,Put、Spd和Spm处理分别提高一年生黑麦草种子的平均发芽率15.5%和12.0%,分别缩短平均发芽时间1.21和1.14d。在种子吸胀期间的耐冷性牧乐8000强于钻石T。总体而言,Put、Spd和Spm处理能提高一年生黑麦草种子吸胀期间的耐冷性,提高低温胁迫下种子发芽能力。[结论]该研究结果为种子引发技术在一年生黑麦草生产上的应用提供了理论依据。
[Objective] The research aimed to discuss the effect of polyamine initiation on physiological and biochemical characters and seed germination of ryegrass embryo. [Method] With annual ryegrass (Diamond T and Makura 8000) as materials, the seeds were induced with 0.5mmol / L Put, Spermidine and Spm for 24h, (12, 24, 36 and 48h). The effects of Put, Spd and Spm on cold tolerance and seed germination ability of annual ryegrass seed were studied. [Result] Put, Spd and Spm priming increased the activity of peroxidase (POD), catalase (CAT), superoxide dismutase (SOD) and ascorbate peroxidase (APX) , And soluble protein content, but also significantly improve the germination rate and shorten the average germination time. Compared with the control, Put, Spd and Spm increased the average germination rate of annual ryegrass by 15.5% and 12.0%, respectively, and shorten the average germination time by 1.21 and 1.14 days, respectively. Cold tolerance during seed swallowed Makoto 8000 is stronger than diamond T. Overall, Put, Spd and Spm treatments improved cold tolerance during annual expansion of annual ryegrass seeds and increased seed germination under low temperature stress. [Conclusion] The results of this study provided the theoretical basis for the application of seed priming technology in annual ryegrass production.