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腐霉根腐病是大豆主要的土传病害,为揭示抗感病大豆品种的生理差异,对不同抗性品种在腐霉病原菌侵染下的大豆下胚轴中丙二醛、苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、可溶性糖、脯氨酸含量的变化进行测定。结果表明:腐霉菌侵染后,抗病大豆品种下胚轴的丙二醛含量增加不十分明显;可溶性糖含量都是正向增加,苯丙氨酸解氨酶活性,过氧化物酶活性,脯氨酸含量在不同时段都有所增加且抗病品种明显高于感病品种。而感病品种在腐霉菌侵染条件下,大豆下胚轴中丙二醛含量累积较高,可溶性糖在某一时段内有所减少,苯丙氨酸解氨酶活性、过氧化物酶活性,脯氨酸含量增加幅度小于抗病品种,表明抗病品种具有较强的生理生化调节能力。
Pythium root rot is the major soil-borne disease of soybean. In order to reveal the physiological differences of soybean cultivars susceptible to susceptible diseases, malondialdehyde, phenylalanine (PAL), peroxidase (POD), soluble sugar, proline content were measured. The results showed that the content of malondialdehyde in hypocotyls of resistant soybean was not significantly increased after inoculation with Pythium, the content of soluble sugar increased positively, the activity of phenylalanine ammonia lyase, the activity of peroxidase, The content of amino acid increased at different time and the resistant varieties were significantly higher than the susceptible varieties. In susceptible varieties, the accumulation of malondialdehyde in hypocotyls of soybean was higher under the infection of Pythium, the soluble sugar decreased in a certain period of time, and the activities of phenylalanine ammonia lyase and peroxidase , Proline content increased less than the resistant varieties, indicating that resistant varieties have strong physiological and biochemical regulatory capacity.