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目的 评价热双蒸水 (DDW)灭瘤及右旋糖酐 40 (Dextran 40 )抗瘤细胞粘附的体外效果。方法 分别用温热DDW ,温热DDP(阳性对照 )及NS(阴性对照 ) ,浸泡B16 F10 瘤细胞 10min及 2 0min ,2 4hr后行活细胞计数。在处理过的培养板孔内分别加Dextran 40及PBS(对照组 ) ,接种B16 F10 细胞 ,16hr后收集贴壁活细胞计数。结果 浸泡 10min时与阴性对照组比 ,温热DDW组和阳性对照组活细胞数均显著减少 (P<0 .0 1) ,后者减少更明显 (P <0 .0 5 ) ;但浸泡 2 0min后 ,两组均无细胞存活 (无差异 )。与对照组比 ,Dextran 40可部分抑制B16 F10 细胞对胶化培养板孔壁的粘附 (P <0 .0 1)。结论 温热DDW浸泡 2 0min能完全杀灭体外培养的B16 F10 细胞。Dextran 40可部分抑制B16 F10 细胞对胶化培养板孔壁的粘附。
Objective To evaluate the in vitro effect of hot double-distilled water (DDW) oncolytic and dextran 40 (Dextran 40) anti-tumor cell adhesion. Methods B16 F10 tumor cells were immersed in warm DDW, warm DDP (positive control) and NS (negative control) respectively for 10 min and 20 min, and viable cells were counted after 24 hr. Dextran 40 and PBS (control group) were added to the treated plate wells, and B16 F10 cells were inoculated. After 16 hours, adherent viable cell counts were collected. Results Compared with the negative control group, the number of viable cells in the warm DDW group and the positive control group decreased significantly (P <0. 01) at 10 min after soaking, while the latter decreased more significantly (P <0. 05) After 0 min, there was no cell survival in both groups (no difference). Compared with the control group, Dextran 40 partially inhibited the adhesion of B16 F10 cells to the wall of the gelatinized culture plate (P <0.01). Conclusion Warm DDW immersion 20min can completely kill B16 F10 cells cultured in vitro. Dextran 40 partially inhibits the adhesion of B16 F10 cells to the wall of the gelatinized culture plates.