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为了深入研究麦冬活性部位体内药效物质基础,本研究应用UHPLC-LTQ-Orbitrap MS结合高能碰撞诱导裂解技术(HCD)对大鼠口服麦冬甾体皂苷后的入血成分进行快速分析,对比给药后大鼠血浆、大鼠空白血浆、麦冬甾体皂苷提取物以及对照品之间的色谱和质谱信息,辅以特征诊断离子判别,最终共鉴定了31个血中移行成分,包括13个呋甾烷醇型甾体皂苷和18个(异)螺甾醇型甾体皂苷。其中,8个移行成分的结构可通过与对照品比对被准确鉴定。该结果可为进一步研究麦冬药效物质基础和作用机制提供依据。
In order to further study the active substances in the body parts of Radix Ophiopogon japonicus in vivo, UHPLC-LTQ-Orbitrap MS combined with high-energy collision-induced lysis (HCD) in rats Ophiopogon japonicas glycosides after oral administration of rapid analysis of components, compared After administration, the chromatograms and mass spectra of rat plasma, rat plasma, Ophiopogon japonicus steroidal saponin extract, and reference substance, together with the characteristic diagnosis of ion discrimination, were finally identified in 31 blood migratory components including 13 A furostanol-type steroidal saponin and 18 (iso) spirosteroid-type steroidal saponins. Among them, the structure of 8 transitional components can be accurately identified by comparison with the reference substance. The results provide the basis for further study on the basis and mechanism of action of Radix Ophiopogonis.