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目的:探讨中药金安对C57BL/6J小鼠Lewis肺癌的抑制效应及其作用机制。方法:荷Lewis肺癌的C57BL/6J小鼠分为生理盐水组、金安高、中、低剂量组和环磷酰胺组,通过检测各组小鼠的体质量变化和抑瘤率,应用流式细胞仪和TUNEL等方法观察金安对Lewis肺癌的抑瘤机制。结果:金安高、中、低剂量组体质量均明显增加,且金安高、中、低剂量组与环磷酰胺组的抑瘤率依次为45.79%,40.90%,32.48%和98.96%,流式细胞仪检测金安各组肿瘤细胞凋亡率分别为24.19%,14.95%和13.93%,并经统计学分析发现金安诱导肿瘤细胞的凋亡率与剂量之间存在依赖关系。TUNEL检测各组凋亡指数(AI)依次为0.79±1.60,6.43±5.62,4.00±7.15,1.14±1.90,经方差分析知金安高剂量组与NS组差异有显著性意义。结论:金安对Lewis肺癌生长具有一定的抑制作用,且促进肿瘤细胞凋亡可能是金安治疗Lewis肺癌的主要作用方式。
Objective: To investigate the inhibitory effect and its mechanism of Chinese medicine Jin’an on Lewis lung carcinoma in C57BL / 6J mice. Methods: C57BL / 6J mice bearing Lewis lung cancer were divided into normal saline group, high, medium and low dose Jin’an group and cyclophosphamide group. The body weight and tumor inhibition rate of mice in each group were detected by flow cytometry Cytometry and TUNEL and other methods to observe Jin’an on Lewis lung cancer inhibition mechanism. Results: The body mass of Jin’an high, medium and low dose groups were significantly increased, and the tumor inhibition rates of Jin’an high, medium and low dose groups and cyclophosphamide group were 45.79%, 40.90%, 32.48% and 98.96% The apoptosis rate of tumor cells in Jinan group was 24.19%, 14.95% and 13.93% respectively by flow cytometry. And the statistical analysis showed that the apoptosis rate of Jinan-induced tumor cells was dose-dependent. The apoptotic index (AI) of TUNEL group was 0.79 ± 1.60, 6.43 ± 5.62, 4.00 ± 7.15 and 1.14 ± 1.90 respectively. The variance analysis showed that there was significant difference between the high dose group and the NS group. Conclusion: Jin’an can inhibit the growth of Lewis lung carcinoma and promote the apoptosis of Lewis lung carcinoma. It may be the main mode of action of Jin’an in treating Lewis lung carcinoma.