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目的了解2012年北京市怀柔区病毒性腹泻标本中轮状病毒(RV)、诺如病毒(NV)、肠道腺病毒(EadV)和星状病毒(AstV)流行现况,为病毒性腹泻的诊治提供依据。方法 RV采用血清学方法检测,NV、EadV和AstV采用RT-PCR联合毛细管电泳法检测,并进行统计学分析。结果 120例标本总的病毒检出率为38.33%。4种常见腹泻病毒的检出率分别为15.83%、16.67%、0.83%和5.00%。结论 2012年北京市怀柔区病毒性腹泻呈高发态势,其中RV和NV是主要的病原之一,并且主要集中在50岁以上成人。RV和NV感染有明显的季节特征,RV秋冬季为发病高峰,NV则冬季相对高发。
Objective To understand the prevalence of rotavirus (RV), norovirus (NV), intestinal adenovirus (EadV) and astrovirus (AstV) in the viral diarrhea specimens of Huairou District in Beijing in 2012, Diagnosis and treatment to provide the basis. Method RV was detected by serological method, NV, EadV and AstV were detected by RT-PCR combined with capillary electrophoresis, and statistical analysis. Results The total detection rate of 120 samples was 38.33%. The detection rates of 4 common diarrhea viruses were 15.83%, 16.67%, 0.83% and 5.00% respectively. Conclusions In 2012, the viral diarrhea in Huairou district of Beijing showed a high incidence. Among them, RV and NV were one of the major pathogens and mainly concentrated in adults over 50 years of age. RV and NV infections have obvious seasonal characteristics, RV autumn and winter peak incidence, NV is relatively high in winter.