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AIM To evaluale the potential role of P-selectinand anti-P-selectin monoclonal antibody(mAb)in apoptosis during hepatic/renal ischemia-reperfusion injury.METHODS Plasma P-selectin level,hepatic/renal P-selectin expression and cell apoptosiswere detected in rat model of hepatic/ renalischemia-reperfusion injury.ELISA,immunohist-ochemistry and TUNEL were used.Someischemia-reperfusion rats were treated with anti-P-selectin mAb.RESULTS Hepatic/renal function insuffic-iency,up-regulated expression of P-selectin inplasma and hepatic/renal tissue,hepatic/renalhistopathological damages and cell apoptosiswere found in rats with hepatic/renal ischemia-reperfusion injury,while these changes becameless conspicuous in animals treated with anti-P-selectin mAb.CONCLUSION P-selectin might mediateneutrophil infiltration and cell apoptosis andcontribute to hepatic/renal ischemia-reperfusioninjury,anti-P-selectin mAb might be an efficientapproach for the prevention and treatment ofhepatic/renal ischemia-reperfusion injury.
AIM To evaluale the potential role of P-selectin and anti-P-selectin monoclonal antibody (mAb) in apoptosis during hepatic / renal ischemia-reperfusion injury. METHODS Plasma P-selectin level, hepatic / renal P-selectin expression and cell apoptosis detected in rat model of hepatic / renalischemia-reperfusion injury. ELISA, immunohist-ochemistry and TUNEL were used. Methischemia-reperfusion rats were treated with anti-P-selectin mAb. RESULTS Hepatic / renal function insuffic- iency, up- selectin inplasma and hepatic / renal tissue, hepatic / renalhistopathological damages and cell apoptosis were found in rats with hepatic / renal ischemia-reperfusion injury, while these changes became non-conspicuous in animals treated with anti-P-selectin mAb. CONCLUSION P-selectin might be mediateneutrophil infiltration and cell apoptosis andcontribute to hepatic / renal ischemia-reperfusioninjury, anti-P-selectin mAb might be an efficientapproach for the prevention and treatment ofhepatic / renal ischemia- reperfusion injury.