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目的:了解热效应对胃癌耐药细胞株SGC7901/ADM各种多药耐药基因的影响。方法:RT-PCR法检测在不同温度下,对不同药物组的人胃癌耐药细胞株SGC7901/ADM和对照的人胃癌敏感细胞株SGC7901中多药耐药基因1(MDR1)、多药耐药相关蛋白(MRP)、肺耐药相关蛋白(LRP)的mRNA表达。结果:胃癌敏感细胞株SGC7901未检出MDR1、MRP和LRP基因的mRNA,而胃癌耐药细胞株SGC7901/ADM中MDR1增加的倍数最多,MRP次之,LRP无表达。高温43℃下,药物多柔比星(ADM)、5-氟尿嘧啶(5-FU)、紫杉醇(TAX)和顺铂(DDP)组MDR1mRNA表达水平分别下降0.19(P=0.000 1)、0.13(P=0.012 1)、0.15(P=0.000 4)和0.12(P=0.000 4),与未加温组相比差异有统计学意义,P<0.05。高温后5-FU、TAX组MRP的mRNA表达水平均降低0.04(P值分别为0.013 4和0.019 6),而ADM、DDP组差异无统计学意义,P值分别为0.633 5和0.664 1。结论:高温的短期处理对MDR mRNA的表达有明显的抑制作用,对MRP则为部分抑制作用,LRP无表达。
Objective: To investigate the effects of thermal effects on multidrug resistance genes in gastric cancer cell line SGC7901 / ADM. Methods: RT-PCR was used to detect the expression of multidrug resistance-1 (MDR1) and multidrug resistance (MDR1) genes in human gastric cancer cell line SGC7901 / ADM and control human gastric cancer cell line SGC7901 at different temperatures. Related protein (MRP), lung resistance-related protein (LRP) mRNA expression. Results: The mRNA levels of MDR1, MRP and LRP were not detected in gastric cancer cell line SGC7901. However, MDR1 increased most in multidrug resistant gastric cancer cell line SGC7901 / ADM, followed by MRP, but not in LRP. MDR1mRNA expression decreased by 0.19 (P = 0.0001) and 0.13 (P) in drug group ADM, 5-FU, TAX and DDP at 43 ℃ = 0.012 1), 0.15 (P = 0.000 4) and 0.12 (P = 0.000 4) respectively. There was a significant difference between the two groups (P <0.05). After high temperature, mRNA expression of MRP decreased by 0.04 in 5-FU and TAX groups (P = 0.013 4 and 0.019 6, respectively), but there was no significant difference between ADM and DDP groups (P = 0.633 5 and 0.664 1 respectively). Conclusion: Short-term high-temperature treatment significantly inhibits the expression of MDR mRNA and partially inhibits MRP while no expression of LRP.