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microRNA(miRNA)是一类长约20~24 nt的非编码单链小分子RNA.环境毒理学研究表明,当生物暴露于环境化学物质时,会引起相关miRNA表达发生变化,进而调节基因的表达.农药氟虫腈是一种苯基吡唑类杀虫剂,能影响斑马鱼中miR-155的正常表达,但尚未见异位表达的miR-155对斑马鱼细胞影响的研究.本研究明确了异位表达的miR-155对氟虫腈作用下斑马鱼胚胎细胞ZF4存活的影响.结果显示,氟虫腈处理ZF4细胞72 h的IC50值为39.05μmol/L,瞬时转染miR-155 mimic,显著降低了ZF4细胞氟虫腈暴露的存活率.同时real-time PCR结果显示,其潜在靶基因cyb561d2表达显著降低;而转染miR-155 inhibitor,抑制细胞内源性的miR-155则可显著增强ZF4细胞抵抗氟虫腈细胞毒性的能力,同时cyb561d2表达则显著增高.以上结果表明,miR-155参与氟虫腈毒理学效应,其表达量的高低可以影响氟虫腈作用下ZF4细胞的存活率,而其可能的途径之一是通过潜在靶基因cyb561d2的表达调控来实现.因此,miR-155可作为氟虫腈环境毒性的潜在的生物标志物,有望成为致癌环境化学物质对生物体作用机制研究的新途径.
MicroRNAs (miRNAs) are a class of non-coding single-stranded small RNAs ranging in length from about 20 to 24 nt. Environmental toxicology studies have shown that when a biological is exposed to environmental chemicals, it can cause changes in the expression of the relevant miRNAs, thereby regulating gene expression Pesticide Fipronil is a phenylpyrazole insecticide that can affect the normal expression of miR-155 in zebrafish, but has not yet seen the ectopic expression of miR-155 on zebrafish cells.This study is clear The effect of ectopic expression of miR-155 on the survival of zebrafish embryos ZF4 cells treated with fipronil showed that the IC50 value of fipronil treatment for 72 h was 39.05 μmol / L, and the transient expression of miR-155 mimic , Significantly reduced the survival rate of fipronil exposure in ZF4 cells.At the same time, real-time PCR results showed that the potential target gene cyb561d2 expression was significantly reduced; while transfected with miR-155 inhibitor, inhibition of cell endogenous miR-155 Significantly enhanced the ability of ZF4 cells to resist the cytotoxicity of fipronil while the expression of cyb561d2 was significantly increased.The above results show that miR-155 is involved in the toxicological effects of fipronil and the expression levels of miR-155 can affect the effect of fipronil on ZF4 cells Survival rate, and its possible ways First, through the regulation of cyb561d2, a potential target gene, miR-155 can be used as a potential biomarker for the environmental toxicity of fipronil and is expected to become a new approach to study the mechanism of action of carcinogenic chemicals on organisms.