玉米细菌性枯萎病是玉米上的重要种传病害,病原菌为Pantoea stewartiisubsp.stewartii。本研究设计了16S通用引物,扩增该病菌及其近似种的16S rDNA,通过序列测定和分析,针对该病菌设计了特异性引物,采用nested-PCR技术,能够准确地区别该病菌及其近似种,检测的灵敏度在DNA水平上达到10-3pg级,检测活菌则达到2 cfu。检测人工污染的玉米种子时,不受种子提取液中其它物质的干扰,灵敏度依然达到2 cfu。 Bacterial wilt of corn is an important disease in maize and the pathogen is Pantoea stewartiisubsp.stewartii. In this study, a 16S universal primer was designed to amplify the 16S rDNA of this bacterium and its approximate species. According to the sequence analysis and analysis, specific primers were designed for this bacterium. Using nested-PCR, Species, detection sensitivity at the DNA level of 10-3pg level, the detection of viable reached 2 cfu. The detection of artificially contaminated corn seeds, the interference of other substances in the seed extract, the sensitivity is still up to 2 cfu.