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建立了采用异相成核和可控水解相结合的方法制备由纳米纤维构建的聚合物微球的新方法.根据多取代卞叉山梨醇(TM6)对聚己二酸丁二酯(PBA)的异相成核作用以及结晶形态的影响,采用两种乳液溶剂挥发法、并在聚合物溶液中加入成核剂TM6制备了PBA微球,研究了成核剂含量对PBA微球在酶促降解之后形态变化的影响.研究结果表明,二次乳液溶剂挥发法可以制备具有多孔结构的PBA微球,这种多孔结构有利于酶溶液进入到微球内部,促进PBA微球的均匀水解,最终获得由PBA纳米纤维构建的微球.当TM6的含量为3 wt%时,采用二次乳液溶剂挥发法制备的PBA微球,经过5 h酶促降解处理,可以得到表面和内部由PBA纳米纤维均匀组成的微球.进一步的细胞实验表明,微球的纳米纤维结构,有利于MG-63细胞在聚合物微球上的黏附、铺展和向内生长.
A new method for preparing polymer microspheres made of nanofibers by heterogeneous nucleation and controlled hydrolysis was established.According to polybutylene terephthalate (PBA) Heterogeneous nucleation and crystallization morphology of the PBA microspheres were investigated by using two emulsion solvent evaporation methods and adding TM6 into the polymer solution to prepare the PBA microspheres. The results showed that PBA microspheres with porous structure could be prepared by the solvent evaporation method of secondary emulsion. This porous structure was favorable for the enzyme solution to enter into the microspheres and promote the uniform hydrolysis of the PBA microspheres. Finally, PBA nanofiber microspheres were prepared.When TM6 content of 3 wt%, using secondary emulsion solvent evaporation PBA microspheres prepared after 5 h enzymatic degradation treatment can be obtained on the surface and inside of the PBA nanofibers Uniform microspheres. Further experiments showed that the nanofibers structure of microspheres favored the adhesion, spreading and in-growth of MG-63 cells on the polymer microspheres.