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目的:观察垂盆草总黄酮(SSTF)对CCL_4诱导肝纤维化大鼠肝组织TGF-β1和Smad7蛋白及mRNA表达的影响。方法:60只雄性SD大鼠随机分为正常组、模型组,SSTF低剂量组(100 mg·kg~(-1))、SSTF中剂量组(200 mg·kg~(-1)),SSTF高剂量组(400 mg·kg~(-1))和秋水仙碱阳性药对照组,共6组,每组10只。CCl_4皮下注射诱导大鼠肝纤维化模型,同时SSTF灌胃干预,7周后处死大鼠。采用Masson染色法检测大鼠肝脏组织病理学改变,Western blotting法检测肝脏组织TGF-β1和Smad7蛋白表达实时定量RT-PCR检测肝脏组织TGF-β1和Smad7mRNA表达。结果:与模型组比较,SSTF各剂量均能显著降低CCL_4诱导的肝纤维化程度(P<0.05);中高剂量可以显著减少肝组织中TGF-β1蛋白和mRNA表达(P<0.05),并且明显增加Smad7蛋白和mRNA表达(P<0.05)。结论:SSTF可有效地逆转实验性大鼠肝纤维化,其机制可能与下调肝组织中TGF-β1蛋白和mRNA表达及增加Smad7蛋白和mRNA表达有关。
Objective: To observe the effect of SSTF on the protein and mRNA expression of TGF-β1 and Smad7 in liver of CCL-4-induced hepatic fibrosis rats. Methods: Sixty male Sprague Dawley rats were randomly divided into three groups: normal group, model group, low dose SSTF group (100 mg · kg -1), middle dose of SSTF group (200 mg · kg -1), SSTF High dose group (400 mg · kg -1) and colchicine positive control group, a total of 6 groups, 10 in each group. CCl 4 subcutaneously induced rat hepatic fibrosis model, while SSTF intragastric administration, 7 weeks after the rats were sacrificed. Masson staining was used to detect the histopathological changes in rat liver. Western blotting was used to detect the expression of TGF-β1 and Smad7 in liver tissue. Real-time quantitative RT-PCR was used to detect the expression of TGF-β1 and Smad7 in liver tissue. Results: Compared with the model group, all doses of SSTF could significantly reduce the degree of hepatic fibrosis induced by CCL_4 (P <0.05); medium and high dosages of TGF-β1 significantly decreased the expression of TGF-β1 protein and mRNA (P <0.05) Increased Smad7 protein and mRNA expression (P <0.05). Conclusion: SSTF can effectively reverse experimental liver fibrosis in rats. The mechanism may be related to the down-regulation of TGF-β1 protein and mRNA expression and the increase of Smad7 protein and mRNA expression in liver tissue.