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调查和测定了中国西北荒漠地区的蒙古沙拐枣(CalligonummongolicumTurcz)5个种群的形态学指标,应用方差分析(MANOVA)、主成分分析(PCA)和聚类分析,对种群间及种群内的形态性状进行研究。结果表明,种群间的形态变异差异显著。植株高度、种子刺毛长度和种子重量指标具有较强的形态差异性分析意义。形态差异(欧氏距离)与种群间基因流动(地理距离)间没有发现相关关系。聚类分析结果表明,种群M1与M2亲缘关系最近,种群M5与其他种群的亲缘关系相对较远;采用垂直板聚丙烯酰胺电泳技术对5个种群的3个酶系统进行了遗传多样性分析,结果表明,种群M5与其他种群的遗传一致度稍低(0.8197～0.8902)以外,其余各种群遗传一致度较高(0.8480～0.9505),体现其亲缘关系较近。依据Nei的遗传距离进行聚类,结果与形态聚类结果有一定的相似性。
The morphological indices of Calligonum mongolicum Turcz in the desert area of northwest China were investigated and determined. The morphological characteristics of five populations of Calligonum mongolicum Turcz were investigated using analysis of variance (MANOVA), principal component analysis (PCA) and cluster analysis. Traits research. The results showed that there was significant difference in morphological variation among populations. Plant height, seed length and seed weight index have strong morphological significance of the analysis. No correlation was found between morphological differences (Euclidean distance) and gene flow among populations (geographic distance). The results of cluster analysis showed that the genetic relationship between M1 and M2 was the closest, and M5 was relatively distant from other populations. The genetic diversity of 3 enzyme systems of 5 populations was analyzed by vertical plate polyacrylamide electrophoresis, The results showed that the genetic identity between M5 and other populations was slightly lower (0.8197-0.8902), while the genetic diversity among the remaining populations was higher (0.8480-0.9505), indicating that the genetic relationship was closer. According to the genetic distance of Nei clustering, the results have some similarities with morphological clustering results.