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目的:建立毛细管区带电泳法测定元胡止痛片和元胡止痛口服液中延胡索乙素的含量。方法:0.2mol·L~(-1)Tris-45mmol·L~(-1)磷酸二氢钠(磷酸调pH 5.30)-40％异丙醇为缓冲液,分离电压为29 kV,检测波长200nm,50cm×50μm,末涂层石英毛细管,每次进样前分别用0.1 mol·L~(-1)的盐酸、氢氧化钠和缓冲液冲洗5 min; 进样方式:压力进样2s。操作温度:25℃。结果:延胡索乙素在0.04～1.60mg·mL~(-1)范围呈线性,RSD小于2.9％,回收率大于97％。结论:实验结果表明,该方法简便、快速、结果准确可靠,有较好的重复性。
Objective: To establish a capillary zone electrophoresis method for the determination of tetrahydropalmatine in Yuanhuzhitong Tablets and Yuanhu Zhitong Oral Liquid. METHODS: 0.2 mol·L -1 Tris-45 mmol·L -1 sodium dihydrogen phosphate (pH 5.30 adjusted with phosphoric acid) -40% isopropanol as buffer, separation voltage 29 kV, detection wavelength 200 nm , 50cm × 50μm, the end of the coating of quartz capillary, respectively, before each injection with 0.1mol · L -1 hydrochloric acid, sodium hydroxide and buffer rinse 5min; injection mode: pressure injection 2s. Operating temperature: 25°C. Results: Tetrahydropalmatine was linear in the range of 0.04 to 1.60 mg·mL -1 , RSD was less than 2.9%, and the recovery was greater than 97%. Conclusion: The experimental results show that this method is simple, rapid, accurate and reliable, and has good repeatability.