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目的 :研究蓖麻凝集素 ( RCA)协同乐铂 ( Lob)对人非小细胞肺癌细胞的杀伤作用。方法 :运用MTT法和间接免疫荧光法 ,分别测定原代培养非小细胞肺癌细胞在 Lob、RCA、RCA+ Lob的作用下生长增殖的情况。结果 :RCA+ Lob组细胞生长抑制率 ( 4 5 .0± 8.4) %比 Lob组 ( 1 4.2± 5 .0 ) %明显增高 ( P<0 .0 1 ) ;RCA+ Lob组癌细胞中 PCNA的含量低于对照及 Lob组 ( P<0 .0 1 )。结论 :RCA增强 Lob对癌细胞生长增殖的抑制作用。
Objective: To study the killing effect of castor lectin (RCA) and Lob on human non-small cell lung cancer cells. Methods: MTT assay and indirect immunofluorescence assay were used to determine the growth and proliferation of primary cultured non-small cell lung cancer cells under the action of Lob, RCA and RCA+ Lob. RESULTS: Inhibitory rate of cell growth in RCA+ Lob group was significantly higher than that in Lob group (4.5 ± 8.4)% (P < 0.01); PCNA content in RCA+ Lob group was significantly higher than that in Lob group (P < 0.01). Lower than the control and Lob groups (P < 0.01). Conclusion : RCA enhances the inhibitory effect of Lob on the growth and proliferation of cancer cells.