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用RT-PCR的方法检测了不同来源的肝细胞和不同的临床来源肝组织样本中MTA1表达差异,发现在肝癌细胞HepG2和SSMC-7721中的MTA1表达显著高于正常来源肝细胞L02,在肝癌组织的MTA1表达量是正常对照和癌旁组织的2倍左右,同时其在转移性肝癌的MTA-1表达也高于非转移肝癌。利用信号通路筛选的方法对常见的和肿瘤相关转录因子进行分析,发现MTA1可以显著的增强癌基因RB的表达,同时对抑癌基因p53具有抑制作用。这些结果说明MTA1在肝癌的发生发展以及转移过程中可能扮演着重要的作用。
The expression of MTA1 was detected by RT-PCR in hepatocytes from different origins and from different clinically derived liver samples. The results showed that the expression of MTA1 in hepatocellular carcinoma cells HepG2 and SSMC-7721 was significantly higher than that in normal liver cells L02, Tissue expression of MTA1 is about 2 times higher than that of normal tissues and adjacent tissues, and its expression of MTA-1 in metastatic liver cancer is also higher than that in non-metastatic liver cancer. Using signal transduction screening method to analyze common and tumor related transcription factors, we found that MTA1 can significantly enhance the expression of oncogene RB, and inhibit the tumor suppressor gene p53. These results indicate that MTA1 may play an important role in the development and metastasis of HCC.