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用细胞膜放射性标记和离子交换层析法、Ｃａ￣（２＋）指示剂的分光光谱法和ＡＰＡＡＰ桥联酶标法分别检测Ｍ内ＩＰ＿３、〔Ｃａ￣（２＋）〕ｉ和膜上Ｉａ抗原的表达，研究ＩＰ＿３受体阻断剂肝素（Ｈ）和钙调蛋白（ＣａＲＰ）抑制剂三氟啦嗪（ＴＦＰ）对ＮＥ调控ＭＩａ抗原表达的影响。结果显示：Ｈ（４０μｇ／ｍｌ）不影响ＮＥ（１０￣（－８）ｍｏｌ／Ｌ）增高Ｍ内ＩＰ＿３的作用（４５２±３０ｃ．ｐ．ｍ．／１０￣６ｃｅｌｌｓ，ＮＥ组：４４２±２２ｃ．ｐ．ｍ．／１０￣６ｃｅｌｌｓ，Ｐ＞０．０５）；但能阻断ＮＥ增高Ｍ〔Ｃａ￣（２＋）〕ｉ的作用（１１４±４３ｎｍｏｌ／Ｌ，ＮＥ组：３２２±７６ｎｍｏｌ／Ｌ，Ｐ＜０．０１）；还能阻断ＮＥ促进ＭＩ－Ａ、Ｉ－Ｅ表达的效应（４６％±４％、４５％±７％，ＮＥ组：６４％±８％、５８％±６％，Ｐ＜０．０１）。ＴＦＰ（５０μｍｏｌ／Ｌ）不影响ＮＥ升高Ｍ〔Ｃａ￣（２＋）〕的作用（３２８±８９ｎｍｏｌ／Ｌ，ＮＥ组：３２２±７６ｎｍｏｌ／Ｌ，Ｐ＞０．０５），却能阻断ＮＥ促进ＭＩ－Ａ、Ｉ－Ｅ表达的效应（４５％±４％、４４％±５％，ＮＥ组：６４％±８％、５８％±６％，Ｐ＜０．０１）。结果提示：ＮＥ调控ＭＩａ抗原表达的效?
The expression of IP_3, [Ca ~ (2 +)] i and the membrane Ia antigen in M were detected by radioimmunofluorescence and ion exchange chromatography, Ca ~ (2+) indicator spectrophotometry and APAAP bridged enzyme labeling To investigate the effects of IP3 receptor blocker heparin (H) and calmodulin (TFP) inhibitor on the NE-mediated MIa antigen expression. The results showed that H (40μg / ml) did not affect NE (10 ~ (-8) mol / L) increased IP3 in M (452 ± 30c.p.m./10 ~ 6cells, NE group: 442 ± 22c. but could block the effect of NE on M (Ca ~ (2 +)] i (114 ± 43nmol / L, NE: 322 ± 76nmol / L, P <0.01), but also blocked the effect of NE on the expression of MI-A and I-E (46% ± 4%, 45% ± 7%, NE: 64% ± 8%, 58% ± 6% P <0.01). TFP (50μmol / L) did not affect the effect of NE on M 〔Ca 2+〕 (328 ± 89nmol / L, NE group: 322 ± 76nmol / L, P> 0.05) MI-A, I-E expression (45% ± 4%, 44% ± 5%, NE group: 64% ± 8%, 58% ± 6%, P <0.01). The results suggest that NE regulates the expression of MIa antigen?