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报道了砹—211和碘—131标记蛋白质的方法.通过过氧化氢或氯胺T直接氧化制备,使用凝胶色谱从反应产物中分离标记蛋白质,相对法比较放射性计数测定标记产率,过氧化氢适用于~(211)At标记蛋白质,氯胺T有利于~(131)I标记蛋白质.8次实验的结果表明,用过氧化氢氧化标记的~(211)At牛血清白蛋白产率96.4％,氯胺T氧化标记的~(?)I牛血清白蛋白产率66.1％.间接法标记蛋白质,放射性核素~(211)At同对氨基苯甲酸反应获得对—~(211)At—苯甲酸,经乙醚萃取和高效液体色谱分离,然后再偶联到免疫球蛋白或牛血清白蛋白上。动物实验结果表明,此种结合的标记蛋白质在体内稳定,标记率不低于初始~(211)At放射性活度的40%.
A method for labeling 砹-211 and iodine-131 labeled proteins was reportedly prepared by direct oxidation of hydrogen peroxide or chloramine T. The labeled protein was separated from the reaction product by gel chromatography and radiolabelled by a relative method to determine the yield of the label. Hydrogen is suitable for the ~ (211) At marker protein, and chloramine T is favorable for the ~ (131) I marker protein.The results of the eight experiments showed that the yield of ~ (211) At bovine serum albumin oxidized with hydrogen peroxide was 96.4 %, And the yield of ~ (?) I bovine serum albumin (TBA) labeled by chloramine T was 66.1% .After indirect reaction of protein and radionuclide ~ (211) At with p-aminobenzoic acid, Benzoic acid, extracted with ether and purified by high performance liquid chromatography before being coupled to immunoglobulins or bovine serum albumin. The results of animal experiments show that the bound labeled protein is stable in vivo with a labeling rate of not less than 40% of the initial ~ (211) At radioactivity.