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为了明确1组在中温下(30℃)高效分解木质纤维素的复合菌系的菌群组成,研究复合菌系预处理秸秆对沼气发酵的影响,利用平板分离法和变性梯度凝胶电泳(DGGE)法研究了中温木质纤维素降解复合菌系BYND-8的菌种组成多样性,通过添加该复合系菌液到以牛粪为原料的沼气发酵体系,研究了添加秸秆降解液对沼气产量的影响.利用平板法分离得到了6株细菌,它们与Serratia sp.PSGB 13、Serratia marcescens strain UFLA-25LS、Serratia marcescens strain DAP33、Alcaligenes sp.YcX-20、Stenotrophomonas maltophilia strain C6和Bacillus cereus isolate BRL02-71的相似率分别达到了99%、100%、96%、100%、100%和99%.同时利用DGGE方法还检测到了1株利用平板分离法没有获得到的细菌,它的16S rDNA V3区的序列与Uncultured bacterium clone ATB-KS-1446具有100%的同源性.稻秆经复合菌系BYND-8预处理后应用于沼气发酵中,在发酵的前15d内,累积产气量达到13 167 mL,甲烷产量达到7 248 mL,比对照分别提高了44.5%和95.3%.复合菌系具有较高的菌种组成多样性,将复合菌系应用于沼气发酵的原料预处理过程中,可以将产气时间提前,并提高产气量.
In order to clarify the microbial community composition of lignocellulose-degrading bacteria in medium temperature (30 ℃), the effect of pretreated straw with biochar on biogas fermentation was studied. The plate separation and denaturing gradient gel electrophoresis (DGGE) ) Method was used to study the bacterial diversity of BYND-8 medium-temperature lignocellulose-degrading bacterial strain. By adding this bacterial strain into the biogas fermentation system using cow dung as raw material, the biogas production by adding straw degradation liquid Six strains of bacteria were isolated by the plate method and they were mixed with Serratia sp. PSGB 13, Serratia marcescens strain UFLA-25LS, Serratia marcescens strain DAP33, Alcaligenes sp. YcX-20, Stenotrophomonas maltophilia strain C6 and Bacillus cereus isolate BRL02-71 The similarity of 16S rDNA V3 region was 99%, 100%, 96%, 100%, 100% and 99%, respectively.And at the same time using DGGE method also detected a bacteria not obtained by plate separation method, its 16S rDNA V3 region The sequence was 100% homologous to that of Uncultured bacterium clone ATB-KS-1446. Rice straw was pretreated with compound strain BYND-8 and applied to biogas fermentation. During the first 15 days of fermentation, The amount of methane reached to 7 248 mL, which was 44.5% and 95.3% higher than that of the control respectively.Composing bacteria strains had higher diversity of bacterial species, and the complex strains were applied to the pretreatment of raw materials for biogas fermentation In the gas production time can be advanced, and increase gas production.