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对195例临床可疑结核病经常规培养和涂片抗酸染色阴性的标本行PCR技术检测。结果51例结核杆菌DNA呈阳性(26.2)144例阴性。阳性患者行抗结核治疗有效;阴性患者随访50例.未见假阳性、假阴性。提示应用PCR检测结核杆菌较常规方法具有准确、灵敏的特点,在结核病诊断中有重要意义。
195 cases of clinical suspicious TB were routinely cultured and smear negative acid-negative specimens detected by PCR technology. Results 51 cases of Mycobacterium tuberculosis DNA was positive (26.2), 144 cases were negative. Positive patients treated with anti-TB effective; negative patients were followed up 50 cases. No false positive, false negative. Prompt application of PCR detection of Mycobacterium tuberculosis than conventional methods with accurate and sensitive features, in the diagnosis of tuberculosis is of great significance.