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根据基因库中已发表的安祖花细菌性疫病致病菌粉黛黄单胞杆菌(Xanthomonas campestris pv.dieffenbachiae)基因序列设计引物XcF1/XcR1、XcF2/XcR2,通过扩增条件优化,建立了安祖花细菌性疫病的Nested-PCR检测方法,可以直接从感染细菌性疫病的安祖花组织DNA中扩增出1条343bp的特异性条带。分析表明该序列完全存在于GenBank登录的Xanthomonas campestris pv.dieffenbachiae序列X70380.1中。通过对安祖花植株进行Nested-PCR扩增,在植株不同部位均检测到了343bp的特异性条带,且病原菌在植株中分布不均匀,其中老叶叶柄中扩增强度最高,根茎连接处、老叶、嫩叶柄、嫩叶、佛焰苞柄扩增强度次之,根、佛焰苞和花序中扩增最弱。
The primers XcF1 / XcR1 and XcF2 / XcR2 were designed according to the sequence of the Xanthomonas campestris pv.dieffenbachiae, a pathogen of the bacterial disease in the anopheles, which was published in the GenBank. Anzou Nested-PCR detection of bacterial blight can amplify a 343 bp specific band directly from the DNA of Anthurium andraeanum infected with bacterial diseases. Analysis showed that the sequence is completely present in GenBank registered Xanthomonas campestris pv.dieffenbachiae sequence X70380.1. By Nested-PCR amplification of Anthurium, 343bp specific bands were detected in different parts of the plant, and the pathogens were unevenly distributed in the plants, among them, the intensity of amplification in the petiole of the old leaves was the highest, The old leaves, young petioles, young leaves, spathe bud strength of the second expansion, the roots, spathe and inflorescence amplification of the weakest.