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目的利用本实验室丰富的内生真菌资源筛选出具有良好生物防治铁皮石斛软腐病的菌株,为开发内生真菌生物防治技术和大田应用打下基础。方法采用内生真菌与软腐病原菌PDA平皿对峙实验确定的18株内生真菌用于生物防治实验,先采用瓶苗共培养的方法确定对铁皮石斛苗致病力弱或具有促生长作用的菌株用于盆栽苗实验,进而确定内生真菌的生物防治效果。结果通过盆栽苗生物防治软腐病病原菌终极腐霉的实验研究,利用统计学分析确定了2株具有良好生物防治效果的内生真菌菌株4829和3952,铁皮石斛苗的存活率分别达到66.7%和60.5%。结论石斛属植物内生真菌具有良好的生物防治铁皮石斛软腐病效果。
Objective To screen out strains with good biological control of soft rot of Dendrobium candidum by using the abundant resources of endophytic fungi in our laboratory and lay the foundation for the development of bio-control technology of endophytic fungi and field application. Methods Endophytic fungi and soft rot pathogen PDA plate confrontation experiments identified 18 endophytic fungi used in biological control experiments, the first bottle seedling co-culture method to determine Dendrobium candidum seedlings weak or have a role in promoting growth of the strain For potting seedlings experiments, and then determine the biological control effect of endophytic fungi. Results The results showed that two endophytic fungi strains 4829 and 3952 with good biocontrol efficacy were determined by means of statistical analysis and the viability of Dendrobium candidum seedlings reached 66.7% 60.5%. Conclusion Dendrobium endophytic fungi have a good biological control of Dendrobium candidum effect.