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目的 研究OK 4 32与IL 2抑制近交系小鼠C57BL/6Lewis肺癌 (LLC)的生长及c fos蛋白的表达。方法 以C57BL/6荷瘤LLC小鼠为模型 ,观察OK 4 32联合IL 2对肿瘤发生、生长转移的影响 ,及用抗鼠c fos单克隆抗体进行SABC免疫组化技术半定量测定c fos在Lewis肺癌原发灶中的表达。结果 二者联用对肿瘤体积和重量的抑制作用明显增强 (P <0 0 1) ,增强抑瘤率 (P <0 0 1) ,肺转移灶数目明显减少。光镜下显示肿瘤灶中出现许多凋亡细胞 ;肿瘤组织电镜观察 ,瘤细胞核染色质浓集成块 ,在核膜内呈新月形或环形排列 ,核膜清楚 ,瘤细胞内质网扩张 ,线粒体肿胀。c fos在联合用药组与对照组比较明显减少 (P <0 0 1)。结论 OK 4 32与IL 2有明显的协同抗肿瘤活性 ,部分原因可能阻抑c fos基因的表达 ;提示OK 4 32联合IL 2可望为临床治疗癌症患者提供一种有效的生物治疗方法。
Objective To investigate the effects of OK 4 32 and IL 2 on the growth and c fos protein expression in murine C57BL / 6 Lewis lung cancer (LLC) inbred mice. Methods C57BL / 6 tumor-bearing LLC mice were used as model to observe the effect of OK 4 32 combined with IL 2 on tumorigenesis, growth and metastasis, and semi-quantitative determination of c fos by SABC immunohistochemistry with anti-mouse c fos monoclonal antibody Lewis lung cancer primary tumor expression. Results The combination of the two drugs significantly inhibited tumor volume and weight (P <0.01), enhanced tumor inhibition rate (P <0.01), and significantly reduced the number of lung metastases. Under the light microscope, many apoptotic cells appeared in the tumor focus. The electron microscopy showed that the chromatin of the tumor cells concentrated into clumps, crescent or annular arrangement in the nuclear membrane, clear nuclear membrane, expansion of the endoplasmic reticulum of the tumor cells, mitochondria swelling. c fos decreased significantly in combination group and control group (P <0.01). Conclusion OK 4 32 and IL 2 have obvious synergistic antitumor activity, which may partly inhibit the expression of c fos gene. It is indicated that OK 4 32 combined with IL 2 is expected to provide an effective biological treatment for patients with cancer.