论文部分内容阅读
目的 :观察尾加压素Ⅱ (UⅡ )对心肌细胞肥大的影响及其与钙离子的关系 ,为防治高血压左室肥厚提供理论依据。方法 :以培养的SD乳鼠心肌细胞为实验模型 ,通过测定心肌细胞表面积、蛋白含量和蛋白合成速率作为心肌细胞肥大的指标 ,应用激光共聚焦显微镜检测UⅡ作用后细胞内游离钙的变化。结果 :①随着UⅡ浓度的增加 ,心肌细胞表面积呈剂量依赖性增加 ,其中 10 -8和 10 -7mol/LUⅡ组心肌细胞表面积分别为 2 0 4 6± 2 6 8和 36 6 2± 2 5 9μm2 ,均明显高于对照组的 (936± 10 0 μm2 ) ,差异非常显著 (P <0 .0 1)。②随着UⅡ浓度的增加 ,心肌细胞的蛋白含量和蛋白合成速率呈递增趋势 ,其中 10 -8和 10 -7mol/LUⅡ组心肌细胞蛋白含量分别为 1.6± 0 .4和 1.9± 0 .3ng/cell,明显高于对照组 (0 .8± 0 .2ng/cell) ,差异非常显著 (P <0 .0 1)。蛋白合成速率分别为 2 5 0 8± 2 99和 2 898± 6 2 5cpm/well,与对照组的 (10 2 6± 92cpm/well)比较 ,差异非常显著 (P <0 .0 1)。③加入 10 -7mol/LUⅡ后心肌细胞内钙离子荧光强度迅速升高 (99.0± 14 .1) ,与对照组 (34.6± 5 .8)相比差异非常显著 (P <0 .0 1)。钙离子浓度由对照组的 (2 88± 15 9)上升至 (95 8± 6 6 4 ) ,二者之间差异亦非常显著 (P <0 .0 1)?
Objective: To observe the effect of urotensin Ⅱ (UⅡ) on cardiomyocyte hypertrophy and its relationship with calcium ion, and to provide a theoretical basis for prevention and treatment of left ventricular hypertrophy of hypertension. Methods: Cardiomyocytes from SD neonatal rats were used as experimental models. The myocardial cell surface area, protein content and protein synthesis rate were determined as indicators of cardiomyocyte hypertrophy. Laser scanning confocal microscopy was used to detect the change of intracellular free calcium concentration. RESULTS: ① With the increase of UⅡ concentration, the cell surface area of myocardial cells increased in a dose-dependent manner. The myocardial cell surface area of 10 -8 and 10 -7mol / LUⅡgroup were respectively 2046 ± 268 and 3662 ± 25 9μm2, were significantly higher than the control group (936 ± 10 0μm2), the difference was significant (P <0.01). ② With the increase of UⅡ concentration, the protein content and protein synthesis rate of cardiomyocytes showed an increasing trend. The protein content of myocardial cells in 10 -8 and 10 -7mol / LUⅡgroup were 1.6 ± 0.4 and 1.9 ± 0.3ng / cell was significantly higher than that of the control group (0.8 ± 0.2 ng / cell), the difference was significant (P <0.01). The rates of protein synthesis were respectively 258 ± 2 99 and 2 898 ± 6 25 cpm / well, which were significantly different from those of the control group (102 6 ± 92 cpm / well) (P <0.01). (3) The fluorescence intensity of intracellular Ca (superscript 2 +) increased rapidly (99.0 ± 14.1) after addition of 10 -7 mol / LUⅡ, which was significantly different from that of the control group (34.6 ± 5.8) (P <0.01). The calcium concentration in the control group increased from (2 88 ± 15 9) to (95 8 ± 6 6 4), and the difference was also significant (P <0.01).