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~(211)At和~(131)I通过氯胺T和过氧化氢直接氧化标记牛血清白蛋白和胰蛋白酶,采用凝胶色谱Sephadex G-75柱洗脱分离蛋白质,γ计数测量和相对比较法计算标记产额。一般认为~(211)At标记蛋白质时使用H_2O_2较好,~(131)I标记蛋白质时则适合使用氯胺T,且蛋白质的结构强烈影响标记结果。在八个不同的实验中,过氧化氢氧化标记的~(211)At-牛血清白蛋白产率为96.9％,氯胺T氧化标记的~(131)I-牛血清白蛋白产率为66.1％。
~ (211) At and ~ (131) I were directly labeled with bovine serum albumin and trypsin by chloramine T and hydrogen peroxide, and the proteins were separated by gel permeation chromatography on a Sephadex G-75 column. Law calculation mark the yield. It is generally believed that ~ (211) At labeled protein is better than H 2 O 2, and ~ (131) I labeled protein is suitable for chloramine T, and the structure of protein strongly influences the labeling results. In eight different experiments, the yield of ~ (211) At-bovine serum albumin oxidized by hydrogen peroxide oxidation was 96.9%, and the yield of 131 I-bovine serum albumin labeled with chloramine T was 66.1 %.