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尽管尚未克隆出卵巢癌特异性肿瘤抑制基因,但在卵巢癌组织细胞中观察到染色体11p缺失,提示11p上可能存在肿瘤抑制基因。应用Southern杂交方法进行杂合子缺失(LOH)分析结果表明,在30%～50%的卵巢癌中存在11p15.5区域的HRAS等位基因缺失。但进一步研究未发现该基因的特异性突变,提示肿瘤抑制基因可能位于HRAS附近的区域,由于这一区域太大,以致于不能克隆候选肿瘤抑制基因。而应用近来研究发现的微卫星重复序列标记可使更加精细地定位染色体缺失区域成为可能,故作者以13个微卫星标记位点对48例浸润性卵巢癌(浆液性癌35例、粘液性癌5例、子宫内膜样
Although ovarian cancer-specific tumor suppressor genes have not yet been cloned, a deletion of chromosome 11p is observed in ovarian cancer cells suggesting that tumor suppressor genes may be present on 11p. Heterozygous deletion (LOH) analysis using Southern blotting showed that there was a deletion of the HRAS allele in region 11p15.5 in 30% to 50% of ovarian cancers. However, further study found no specific mutations in the gene, suggesting that the tumor suppressor gene may be located in the vicinity of HRAS, because this area is too large, so that the candidate tumor suppressor gene can not be cloned. However, using the recently discovered microsatellite repeat markers makes it possible to locate the chromosomal regions more precisely. Therefore, the authors used 13 microsatellite markers to screen 48 cases of invasive ovarian cancer (35 cases of serous carcinoma, 5 cases, endometrial like