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Objective:To investigate the effects of seawater immersion on the function of myocardium and hepatocyte mitochondria in experimental hemorrhagic shock rats.Methods: Twenty-four male Wistar rats were divided into three groups (n = 8 in each group): control group,HSL group (hemorrhagic shock group on land) and HSS group (hemorrhagic shock group in seawater ). The hemodynamic parameters, activities of H + -ATPase( adenosinetriphosphatase ), succinate dehydrogenase(SDH) and Ca2+-Mg2+ -ATPase, the calcium contents in myocardium and hepatocyte mitochondria were measured and the changes of proton translocation across the inner mitochondrial membrane were analyzed.Results: The hemodynamic indexes and the activities of H + -ATPase, SDH, Ca2 + -Mg2 + -ATPase in HSS group were significantly lower than those in control group and HSL group (P ＜0.05). In HSS group the calcium levels in tissue and mitochondria of myocardium and hepatocyte were elevated significantly compared with control group and HSL group(P ＜ 0.05). There was no significant difference in proton translocation among three groups.Conclusions: This investigation demonstrates that seawater immersion can aggravate the conditions of hemorrhagic shock rats.