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以甘肃省3个地理种源沙拐枣幼苗为试材,采用PEG模拟胁迫方法,研究不同浓度PEG胁迫处理对沙拐枣幼苗的脯氨酸(Pro)、可溶性糖(SS)、丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化物酶(POD)等生理指标的影响,并通过隶属函数法对3个地理种源的材料进行抗旱性综合评价。结果表明,3个地理种源幼苗各项指标在不同强度PEG胁迫处理下总体变化趋势基本一致,各处理间差异显著(P<0.05)。各指标在3个地理种源间增加幅度排序分别为Pro含量是武威>酒泉>张掖,SS含量是酒泉>张掖>武威,MDA含量是武威>张掖>酒泉,SOD是武威>张掖>酒泉,而POD活性则是酒泉>武威>张掖。对沙拐枣抗旱性分析发现,3个地理种源沙拐枣的抗旱性顺序为武威>张掖>酒泉。不同地理种源沙拐枣的耐旱机制虽存在差异,但保护酶系统调节机制是其对生态环境适应的生理反应共性。
In this study, seed germination of three provenances of Calligonum calli in Gansu Province were studied. PEG simulated stress was used to study the effects of different concentrations of PEG on proline (Pro), soluble sugar (SS), malondialdehyde (MDA), superoxide dismutase (SOD), peroxidase (POD) and other physiological indicators, and through the membership function method of three geographic provenances of drought resistance comprehensive evaluation. The results showed that the overall trend of the three indicators of seedlings of geographically provenances under the PEG stress treatment was basically the same, with significant differences among treatments (P <0.05). The order of increase of each index among the three geographical provenances was as follows: the content of Pro was Wuwei> Jiuquan> Zhangye, the content of SS was Jiuquan> Zhangye> Wuwei, the content of MDA was Wuwei> Zhangye> Jiuquan, SOD was Wuwei> Zhangye> Jiuquan, while POD activity is Jiuquan> Wuwei> Zhangye. The results showed that the order of drought resistance of Caogi jujube in three geographic provenances was Wuwei> Zhangye> Jiuquan. Although the mechanism of drought tolerance differs in different provenances of Cassia angustifolia, the regulatory mechanism of the protective enzyme system is its common physiological response to the ecological environment.