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目的 :研究蕨类植物半边旗提取物对HL 60细胞的体外杀伤作用及对细胞周期的影响。方法 :用噻唑蓝 (MTT)法测定细胞成活率 ;用流式细胞术测定细胞周期时相分布。结果 :半边旗乙醇总提取物PSE及纯化合物 5F、6F及A对HL 60细胞有强烈的杀伤作用 ,具明显的时间和剂量效应 ;PSE、5F、6F及A作用HL 60细胞 2 4h后 ,G0 /G1期细胞比例明显下降 ,S期细胞比例明显升高 ,G2 /M期轻度升高 ( 5F作用组例外 )。结论 :半边旗提取物对HL 60细胞有强烈的杀伤作用 ,明显影响细胞周期时相分布。
Objective : To study the killing effect of Pteridium flavescens extract on HL 60 cells in vitro and its effect on cell cycle. Methods: The survival rate of cells was measured by MTT method. The phase distribution of cell cycle was determined by flow cytometry. RESULTS: PSE from P. semi-marginata and pure compounds 5F, 6F and A had a strong killing effect on HL 60 cells, with obvious time and dose effects; PSE, 5F, 6F and A acted on HL 60 cells 24 hours later. The proportion of G0/G1 phase cells decreased significantly, the proportion of S phase cells increased significantly, and the G2/M phase increased slightly (except for the 5F effect group). Conclusion: The extract of Pteris semipinnata has a strong killing effect on HL 60 cells, which significantly affects the cell cycle distribution.