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过去作抗酸菌的涂片检查,认为涂片不宜过厚,否则染料会遮盖标本影响检出率。实际上过去采用萋尼氏薄涂片取痰液标本太少,检出率不够高。采用厚涂片法,取痰液标本比薄片量多3～5倍,大约0.1毫升,从而提高了检出率。据北京市结核病防治所报道,330份痰标本同时采用厚涂片、浮游集菌、培养三种方法进行比较,检出率分别是21.9％、11.6％、21.3％,以厚涂片的检出率为最高。我们曾选择肺结核患者痰标本300份,每份标本用萋尼氏抗酸染色(即薄涂片法)和厚涂片抗酸染色两种方法,每种方法作两张痰片,结果是萋尼氏法检出率为27％,而厚涂片检出率为42％,厚涂片法明显高于薄涂片。
In the past for acid-fast smear examination, that the smear should not be too thick, otherwise the dye will cover the impact of specimen detection rate. In fact, the past Niu Shi thin smear sputum samples too little, the detection rate is not high enough. Thick smear method, take sputum specimens than the amount of 3 to 5 times the film, about 0.1 ml, thereby increasing the detection rate. According to the report of Beijing Tuberculosis Prevention and Control Institute, 330 sputum samples were also tested by thick smear, floating bacteria-collecting and culturing methods. The detection rates were 21.9%, 11.6% and 21.3% respectively. The highest rate. We have selected 300 cases of sputum samples of patients with tuberculosis, each specimen with Niño acid staining (thin coating method) and thick smear acid-fast staining of two methods, each method for two sputum, the result is 萋Nissl’s detection rate was 27%, while the thick smear detection rate was 42%, thick smear was significantly higher than the thin smear.