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目的探讨盐酸氨基葡萄糖及其与甲氨蝶呤联用对佐剂性关节炎(AA)大鼠的治疗作用。方法将佐剂性关节炎大鼠分为4组:模型对照组(AA组)、盐酸氨基葡萄糖组(GH组)、甲氨蝶呤组(MTX组)和联合用药组(GHM组),另设1组空白对照。自造模前1d至造模后22d,正常对照组和AA组每天给予蒸馏水灌胃,其他组以相应的药物灌胃。造模前及造模后不同时间,测量各组大鼠左、右后足体积,造模后22d,ELISA法检测大鼠血清中肿瘤坏死因子-α(TNF-α)的含量。结果与AA组比较,在继发反应期,GH、MTX及GHM各组的左、右后足体积和血清中TNF-α含量均有所下降,且差异均有统计学意义,除血清中TNF-α含量GHM组显著低于MTX组外,其他各项指标GHM组与GH组和MTX组比较,差异均无统计学意义。结论盐酸氨基葡萄糖可缓解大鼠佐剂性关节炎症状,具有抗炎作用和治疗类风湿性关节炎的潜力,其与MTX联用,有望降低MTX的用量。
Objective To investigate the therapeutic effect of glucosamine hydrochloride combined with methotrexate on adjuvant arthritis (AA) rats. Methods The adjuvant arthritis rats were divided into four groups: model control group (AA group), glucosamine hydrochloride group (GH group), methotrexate group (MTX group) and combination therapy group (GHM group) Set a blank control group. One day before modeling and 22 days after modeling, normal control group and AA group were given distilled water daily, the other groups were given the corresponding drugs. Before modeling and at different time after modeling, the left and right hind paw volume of rats in each group were measured, and the content of tumor necrosis factor-α (TNF-α) in the serum was detected by ELISA after 22 days. Results Compared with AA group, in the secondary reaction period, the contents of TNF-α in the left and right hind paws of GH, MTX and GHM groups decreased, and the difference was statistically significant. Except serum TNF -α content in GHM group was significantly lower than that in MTX group, and there was no significant difference in other indexes between GHM group and GH group and MTX group. Conclusion Glucosamine hydrochloride can relieve the symptoms of adjuvant arthritis in rats, has the anti-inflammatory effect and the potential of treating rheumatoid arthritis. Combined with MTX, it is expected to reduce the dosage of MTX.