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AIM:To assess the direct effects of ischemia on intestinal epithelial integrity. Furthermore,clinical efforts at mitigating the effect of hypoperfusion on gut permeability have focused on restoring gut vascular function. METHODS:We report that,in the Caco-2 cell model of transepithelial transport,calcium glycerophosphate(CGP),an inhibitor of intestinal alkaline phosphatase F3,has a significant effect to preserve transepithelial electrical resistance(TEER) and to attenuate increases in mannitol flux rates during hypoxia or cytokine stimulation. RESULTS:The effect was observable even at concentrations as low as 1 μmol/L. As celiac disease is also marked by a loss of gut epithelial integrity,the effect of CGP to attenuate the effect of the α-gliadin peptide 31-55 was also examined. In this instance,CGP exerted little effect of preservation of TEER,but significantly attenuated peptide induced increase in mannitol flux. CONCLUSION:It appears that CGP treatment might synergize with other therapies to preserve gut epithelial integrity.
METHODS: We report that, in the Caco-2 cell model of transepithelial transport, calcium glycerophosphate (CGP), an inhibitor of intestinal alkaline phosphatase F3, has a significant effect to preserve transepithelial electrical resistance (TEER) and to attenuate increases in mannitol flux rates during hypoxia or cytokine stimulation. RESULTS: The effect was observable even at concentrations as low as 1 μmol / L. As celiac disease is also marked by a loss of gut epithelial integrity, the effect of CGP to attenuate the effect of the α-gliadin peptide 31-55 was also examined. In this instance, CGP exerted little effect of preservation of TEER, but significantly attenuated peptide induced increase in mannitol flux. CONCLUSION: It appears that CGP treatment might synergize with other t herapies to preserve gut epithelial integrity.