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研究以HPLC测定滨蒿内酯和泽兰内酯,调查37种石斛中滨蒿内酯和泽兰内酯的分布式样。其中,对球花石斛按年龄采集假鳞茎,进行滨蒿内酯和泽兰内酯的含量测定。分析表明,滨蒿内酯和泽兰内酯在石斛属中分布差异较大,只有球花石斛和密花石斛含有滨蒿内酯和泽兰内酯,美花石斛中含有少量泽兰内酯。滨蒿内酯和泽兰内酯的含量随球花石斛植株变老有递减的趋势。HPLC简便、快速、准确、重复性好,适用于分析药用石斛中滨蒿内酯和泽兰内酯的含量。
In this study, scopolactone and Zeonelactone were determined by HPLC. The distribution patterns of scoparone and zelactone in 37 species of Dendrobium were investigated. Among them, the bulb Dendrobium collected by age pseudobulb were scopolallyl and zelactone content determination. The results showed that there was a significant difference in the distribution of scoparidene and zuolactone in the genus Dendrobium, only D. officinale and D. dendrobium contained scopolamine and zuolactone, and D. officinale contained a small amount of zuolactone . Scopolactone and Zeeland lactone content of Dendrobium candidum with aging decreasing trend. HPLC is simple, rapid, accurate and reproducible. It is suitable for the determination of scoparone and zealand lactone in medicinal Dendrobium.