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目的对改良TSA-L培养基进行改进,建立一种配方简单、成本低廉及适合结核分枝杆菌L型(Mycobacterium Tuberculosis L forms,MTB-L)快速生长的培养方法。方法在改良TSA-L培养基中加入最适比例的有利于分枝杆菌生长的卵磷脂(Lecithin)和胆固醇(Cholesterin),制备LC-MTB-L培养基;采用改良TSA-L培养基和LC-MTB-L培养基接种培养肺科门诊患者痰标本,比较两种培养基的MTB-L阳性检出率和培养阳性所需时间。结果 LC-MTB-L培养基中卵磷脂和胆固醇的最适加入量均为0.6 g;LC-MTB-L培养基MTB-L阳性检出率显著高于改良TAS-L培养基(P<0.05),同时其培养阳性所需时间显著低于改良TAS-L培养基(P<0.05)。结论 LC-MTB-L培养基配方简单、成本低廉、MTB-L生长快速,适合临床推广应用。
OBJECTIVE To improve the modified TSA-L medium and establish a culture method with simple formula, low cost and suitable for rapid growth of Mycobacterium tuberculosis L forms (MTB-L). Methods The optimal ratio of Lecithin and Cholesterin to mycobacterial growth was added to modified TSA-L medium to prepare LC-MTB-L medium. The modified TSA-L medium and LC -MTB-L culture inoculated sputum specimens from patients in the outpatient department of lung clinic. The positive rate of MTB-L and the time required for culture positive were compared between the two cultures. Results The optimum dosage of lecithin and cholesterol in LC-MTB-L medium was 0.6 g, and the positive rate of MTB-L in LC-MTB-L medium was significantly higher than that of modified TAS-L medium ), While the time required for culture positive was significantly lower than that of modified TAS-L medium (P <0.05). Conclusion LC-MTB-L medium has the advantages of simple formula, low cost and rapid growth of MTB-L, which is suitable for clinical popularization and application.