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采用2.0 G聚酰胺-胺(PAMAM)、石墨烯(GS)和1-丁基-3-甲基咪唑基四氟硼酸盐复合膜修饰玻碳电极(PAMAM/GS/IL/GCE),包埋固定多环芳烃抗体,构建了一种纳米免疫传感器,用于快速测定粮油食品中的苯并(a)芘(Ba P)。在p H为7.0含1mmol/L K3[Fe(CN)6]和0.1 mmol/L KCl的磷酸盐缓冲溶液中,基于多环芳烃抗体与抗原之间的特异性免疫反应,以K3[Fe(CN)6]为探针,利用循环伏安法和差分脉冲伏安法研究其免疫反应对传感器响应电流的影响。在优化实验条件下,免疫传感器的峰电流随溶液中Ba P浓度的增大而减小,且峰电流与Ba P浓度在0.1~100 ng/m L范围内呈线性关系,其检测限为0.03 ng/m L(S/N=3)。该免疫传感器的稳定性和重现性较好,利用该法对玉米油和菜籽油等样品中的苯并(a)芘进行检测,回收率在94.46%~103.36%,检测结果与HPLC法基本一致,用于粮油食品中苯并(a)芘的快速检测是可行的。
The glassy carbon electrode (PAMAM / GS / IL / GCE) was modified with 2.0 G polyamidoamine (PAMAM), graphene (GS) and 1-butyl-3-methylimidazolium tetrafluoroborate The immobilized polycyclic aromatic hydrocarbons (PAHs) were immobilized to construct a nanosized immunosensor for the rapid determination of benzo (a) pyrene (BaP) in grain and oilseeds. Based on the specific immune response between polycyclic aromatic hydrocarbons (PAHs) and antigens in pK 7.0 phosphate buffer solution containing 1 mmol / L K3 [Fe (CN) 6] and 0.1 mmol / L KCl, CN 6] as probes, the effects of their immune responses on the sensor response current were investigated by cyclic voltammetry and differential pulse voltammetry. Under optimized experimental conditions, the peak current of immunosensor decreased with the increase of BaP concentration in solution, and the peak current was linear with the concentration of Ba P in the range of 0.1-100 ng / mL with a detection limit of 0.03 ng / m L (S / N = 3). The stability and reproducibility of the immunosensor is good. The detection of benzo (a) pyrene in corn oil and rapeseed oil samples with the recovery of 94.46% ~ 103.36% Basically the same, for the rapid detection of benzo (a) pyrene in grain and oil food is feasible.