紫丁香苷为天山雪莲的主要活性成分之一,本研究运用溶剂提取方法,结合各种色谱手段对天山雪莲组织细胞培养物中的紫丁香苷进行分离纯化,并根据现代谱学方法对其结构进行鉴定。同时采用高效液相色谱法建立了天山雪莲组织细胞培养物中紫丁香苷的含量测定方法,该方法简便,精密度、重现性良好,结果准确可靠。这些研究为通过天山雪莲细胞悬浮培养大规模生产活性成分紫丁香苷奠定了基础。 Syringaloside is one of the main active components of Tianshan snow lotus. In this study, the solvent extraction method was used to separate and purify the syringin from Tissue culture of Syringa oblata with various chromatographic methods. Based on modern spectroscopic methods, Identification. At the same time, HPLC method was used to determine the content of syringin in Tissue Culture of Tianshan. The method was simple, accurate and reproducible, and the results were accurate and reliable. These studies provide the basis for the large-scale production of the syringosidin via cell suspension culture of Saussurea involucrata.