In this study, we selected 10 susceptible SNPs loci to investigate their contribution to susceptibility to type 2 diabetes in Han Chinese among Hubei population. We genotyped SNPs rs5219, rs1801282, rs1470579, rs1111875, rs1081661, rs7754840, rs4506565, rs13266634, rs4402960, and rs5643981 by using the method of polymerase chain reaction-ligase detection reaction (PCR-LDR). In a case-control study, we have genotyped the 10 candidate susceptibility SNP loci, and here, we reported that the SNP rs5219 in KCNJ11 was strongly associated with type 2 diabetes in Han Chinese in Hubei China. There were significant differences in the TT genotype frequency (OR=1.6, 95%CI 1.1-2.3, p=0.01) and T allele frequency (OR=1.3, 95%CI 1.1-1.6, p=0.03) of SNP rs5219 between cases and controls. The other nine SNP loci did not show significant association with type 2 diabetes in Han Chinese in Hubei. The result suggests that KCNJ11 gene is a susceptibility gene of type 2 diabetes among this population. We selected 10 susceptible SNPs loci to investigate their contribution to susceptibility to type 2 diabetes in Han Chinese among Hubei population. We genotyped SNPs rs5219, rs1801282, rs1470579, rs1111875, rs1081661, rs7754840, rs4506565, rs13266634, rs4402960, and rs5643981 by using the method of polymerase chain reaction-ligase detection reaction (PCR-LDR). In a case-control study, we have genotyped the 10 candidate susceptibility SNP loci, and here, we reported that the SNP rs5219 was KCNJ11 was strongly associated with type 2 diabetes in Han Chinese in Hubei China. There were significant differences in the TT genotype frequency (OR = 1.6, 95% CI 1.1-2.3, p = 0.01) and T allele frequency (OR = 1.3, 95% CI 1.1-1.6 , p = 0.03) of SNP rs5219 between cases and controls. The other nine SNP loci did not show significant association with type 2 diabetes in Han Chinese in Hubei. The result suggests that KCNJ11 gene is a susceptibility gene of type 2 diabetes among this population .