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本文建立了一个微弹介导的火炬松遗传转化系统。这个系统解决了火炬松遗传转化过程中存在的许多困难。运载抗虫基因的质粒载体经微弹转化法进入火炬松成熟合子胚,然后在添加了卡那霉素的培养基上从转化的成熟胚上诱导出有器官发生潜力的愈伤组织,再从转化的愈伤组织上产生转基因植株。利用这一系统生产的转基因植株已经被随机扩增技术、Southern杂交技术和虫试验所证实,并且转化的植株已在土壤中成活。图3表2参28。
In this paper, a microprojectile-mediated loblolly pine transformation system has been established. This system addresses many of the difficulties in the genetic transformation of Pinus taeda. The plasmid vector carrying the insect-resistant gene enters into the mature embryo of Pinus taeda by micro-bomb transformation, and then the callus with organogenic potential is induced from the transformed mature embryo on the kanamycin-supplemented medium. Transgenic callus produces transgenic plants. Transgenic plants produced using this system have been validated by random amplification techniques, Southern hybridization techniques and insect tests, and the transformed plants have survived in the soil. Figure 3 Table 2 参 28.