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目的:探讨大肠癌组织中胃泌素(GAS)、生长抑素(SS)的表达及其比势与细胞周期调控蛋白P16~(INK4a)、P21~(CIP1)及细胞周期素(Cyclins)、细胞周期依赖性蛋白激酶(CDKs)表达的关系.方法:随机选择79例大肠癌患者的手术切除标本,采用免疫组化SP法检测GAS、SS、P16~(INK4a)、P21~(CIP1)、Cvclin D1、Cvclin E、Cyclin A、Cyclin B1、CDK2、CDK4的表达情况.结果:Cyclin D1、CDK2、CDK4、Cyclin A在GAS高表达组、中表达组的阳性表达率明显高于低表达组;而P16~(INK4a)、P21~(CIP1)阳性表达率与此相反.cyclin E在SS低表达组的阳性表达率明显高于中表达组、高表达组;P21~(CIP1)在ss高表达组、中表达组的阳性表达率明显高于低表达组;CDK2在SS低表达组的阳性表达率明显高于SS高表达组.大肠癌组织中GAS、SS表达的积分比值(GAS/SS)与Cyclin D1(r =0.252)、Cyclin E(r=0.387)、Cyclin A(r= 0.466)、CDK2(r=0.519)、CDK4(r=0.434)呈正相关(P<0.01)与P16~(INK4a)(r=-0.385)、P21~(CIP1)(r =-0.454)蛋白的表达积分呈负相关(P<0.01).结论:GAS、SS对大肠癌细胞生长的调控可能与P16~(INK4a)、P21~(CIP1)、Cvclin D1、Cvclin A、CDK2、CDK4、cvclin E基因异常表达有关.GAS对大肠癌细胞周期的调控位点可能在G1、S、G2期,SS对大肠癌细胞周期的调控位点可能在G1/S、G2/M期的交界点,即S、M期的入口.对大肠癌GAS/SS积分比值分析、可作为临床大肠癌生物学行为的重要评估指标.
Objective: To investigate the expression of gastrin (GAS) and somatostatin (SS) in colorectal carcinoma and the relationship between the specific potentials and the cell cycle regulatory proteins P16 INK4a, P21 CIP1 and Cyclins, (CDKs) in patients with colorectal cancer.Methods: Seventy-nine patients with colorectal cancer were randomly selected for surgical resection and immunohistochemical SP method was used to detect the expression of GAS, SS, P16 INK4a, P21 CIP1, Cvclin D1, Cvclin E, Cyclin A, Cyclin B1, CDK2 and CDK4 were detected by immunohistochemistry.Results: The positive expression rates of Cyclin D1, CDK2, CDK4 and Cyclin A in GAS overexpression group were significantly higher than those in low expression group, The positive expression rate of Cyclin E in SS low expression group was significantly higher than that in middle expression group and high expression group, P21 ~ (CIP1) was highly expressed in ss The positive expression rate of CDK2 in SS low expression group was significantly higher than that in SS high expression group, and the ratio of GAS / SS expression score (GAS / SS) in colorectal carcinoma tissue was significantly higher than that in low expression group There was a positive correlation between Cyclin D1 (r = 0.252), Cyclin E (r = 0.387), Cyclin A (r = 0.466), CDK2 (r = 0.519) and CDK4 (r = 0.434) The expression of P16 INK4a (r = -0.385) and P21 CIP1 (r = -0.454) was negatively correlated (P <0.01) .Conclusion: The regulation of GAS and SS on the growth of colorectal cancer cells may be associated with P16 ~ (INK4a), P21 ~ (CIP1), Cvclin D1, Cvclin A, CDK2, CDK4 and cvclin E. The effect of GAS on the cell cycle of colorectal cancer may be at G1, Colorectal cancer cell cycle control sites may be in the G1 / S, G2 / M phase of the junction, that is, S, M period of entry. Colorectal cancer GAS / SS integral ratio analysis can be used as clinical colorectal cancer biological behavior important Evaluation indicators.