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从恙虫病立克次体（Ｒｔ）Ｋａｒｐ株的群特异性抗原基因序列设计两对引物建立套式ＰＣＲ检测现场鼠体采集的恙螨幼虫体内及恙虫病现症病人外周血单核细胞内的ＲｔＤＮＡ。除一份已用过药病人标本外，其余９份均可检见８８ｂｐＤＮＡ扩增产物；检测结果表明：现场鼠体采集的地里纤恙螨（Ｌｅｐｔｏｔｒｏｍｂｉｄｉｕｍｄｅｌｉｅｎｓｅ）幼虫的Ｒｔ携带率是１３．３％，证实套式ＰＣＲ可用于急性期恙虫病的诊断和媒介恙螨流行病学的调查。
Two pairs of primers were designed based on the sequence of group-specific antigen of Rick Karp strain of tsutsugamushi disease (Rt). Two sets of primers were designed to detect the mumps larvae collected from chick mite larvae in vivo and the pathogens of tsutsugamushi disease in peripheral blood mononuclear cells RtDNA. In addition to a sample of used drug patients, the remaining 9 samples can be detected 88bpDNA amplification products; test results show that: the live rat body collected Chilo hairy chigger (Leptotrombidiumdeliense) larvae Rt carrier rate was 13.3% , Confirmed nested PCR can be used for the diagnosis of acute tsutsugamushi disease and vector chigger epidemiological investigation.