【摘 要】
：
Rhizoctonia cerealis is the main pathogen of wheat sharp eyespot in our country.In order to obtain high quality protoplasts for the transformation of Rhizoctonia cerealis, the effect factors on protop
【机 构】
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Department of Plant Pathology, College of Plant Protection,Shandong Agricultural University ,Tai'an
【出 处】
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中国植物病理学会第十二届青年学术研讨会
论文部分内容阅读
Rhizoctonia cerealis is the main pathogen of wheat sharp eyespot in our country.In order to obtain high quality protoplasts for the transformation of Rhizoctonia cerealis, the effect factors on protoplast preparation of R.cerealis were studied.The results showed that the suitable conditions of protoplast preparation were 6 d mycelium, in the mixture enzymes of 15 mg/mL Lywallayme and 10 mg/mL Snailase, digesting at 30℃ for 4 h, the protoplast yield reached 3.0 × 106 cell/mL.
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