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Reservoir sediment is one of the most stressful environments for microorganisms due to periodically oxygen minimum conditions induced by seasonal thermal stratification.In this study,the abundance and composition of bacteria associated with sediments from three drinking water reservoirs (Zhoucun,ZCR; Shibianyu,SBYR and Jinpen,JPR) were investigated by quantitative PCR and 16S rRNA-based 454 pyrosequencing.The results of physico-chemical analysis of sediments showed that the organic matter and total nitrogen were significantly higher in ZCR as compared to JPR (P<0.01).