【摘 要】
：
Functional loss resulting from spinal cord injury (SCI) can often have permanent devastating effects on patient quality of life.The marine-derived natural cembranolide compound WH was originally isola
【机 构】
：
Doctoral Degree Programin Marine Biotechnology, National Sun Yat-sen University and Academia Sinica,
【出 处】
：
第三届国际神经再生高峰论坛暨第五届脊髓损伤治疗与临床试验国际交流会(INRS2013 & 5th ISCITT)
论文部分内容阅读
Functional loss resulting from spinal cord injury (SCI) can often have permanent devastating effects on patient quality of life.The marine-derived natural cembranolide compound WH was originally isolated from the Formosan soft coral.WH significantly decreases the expression of the pro-inflammatory proteins inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 in activated microglial cells.Moreover,it attenuates neuronal cytotoxicity and apoptosis.The aim of the present study was to determine whether WH is also a neuroprotective agent that can attenuate the progression of SCI-associated neurodegeneration.WH,methylprednisolone,or saline (control) were administered by intrathecal injection to Wistar rats after a thoracic spinal cord contusion injury was induced using a New York University Multicenter Animal Spinal Cord Injury Study impactor.Compared with methylprednisolone or saline treatment,WH-treated animals showed significantly better locomotor function recovery after SCI.In addition,WH attenuated the SCI-induced upregulation of pro-inflammatory proteins (ICAM-1,iNOS,TNF-alpha,and IL-1beta) and provided neuroprotection by activating the survival signaling proteins Akt,ERK,and CREB.Today,it is possible to produce WH by cultivating soft coral in terrestrial tanks for further assessment in pre-clinical trials.In conclusion,these results show that WH exerts anti-apoptotic effects in the early phase and anti-inflammatory effects in the late phase of SCI.We believe this compound may be a promising therapeutic agent for SCI.
其他文献
Alzheimers disease (AD) is a pathology characterized by the presence of too much amyloid-beta (Ap) in the brain and the formation of senile plaques.Beta-site amyloid precursor protein cleaving enzyme
Prevention and treatment of stroke is significant in eiderly populations in Chinese communities.This study was designed to explore the stroke risk in an elderly population in Beijing,China and the pra
In order to objectively evaluate recovery of wrist extension function in patients after stroke using surface electromyography,this study compared the outcomes of different acupuncture methods on funct
The aims of this study were to detect the taurine levels in fetal rat brains with intrauterine growth restriction (IUGR),and to determine the influence of antenatal taurine supplementation on taurine
Astrocytes react to central nervous system injury and participate in gliotic responses,imparting both negative and positive effects on axonal regeneration.Despite the considerable biochemical and morp
Doublecortin (DCX)-positive neural precursor cells (NPCs) comprise a significant phagocytic population within the neurogenic zones during adult neurogenesis,and their phagocytic activity critically co
Valproic acid (VPA) is used as an antiepileptic drug or mood stabilizer.Recent studies have shown that exposure to VPA during embryonic development alters neural progenitor cell proliferation,and can
Hirschsprungs disease is a congenital condition characterised by the absence of enteric ganglia and is often associated with neurological abnormalities.This disease in rats,as in human,can be caused b
Spinal cord injury elicits an inflammatory response that plays an important role during the pathological response process by activating the resident microglia and infiltrating macrophages.Macrophages
There is strong evidence that spinal 5-HT3 receptor plays a facilitatory role in various pain states.Electrophysiological studies demonstrated that spinal 5-HT3 receptor is involved in nociception ind