【摘 要】
：
目的 观察刺五加(Acanthopanax senticosus)注射液对小鼠密闭缺氧和人脐静脉内皮细胞(ECV304)常压缺氧的保护作用.方法 以KM清洁级雄性小白鼠和ECV304为研究对象,通过小鼠密闭缺氧,记录刺五加高(25 ml/kg)低(12.5 ml/kg)剂量组小鼠因缺氧而死亡的时间；细胞培养,MTT法检测刺五加高(100 μL/mL)低(50 μL/mL)剂量组作用48 h后波长4
【机 构】
：
新乡医学院,新乡453003;军事医学科学院基础医学研究所,北京100850 新乡医学院,新乡45
【出 处】
：
中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
论文部分内容阅读
目的 观察刺五加(Acanthopanax senticosus)注射液对小鼠密闭缺氧和人脐静脉内皮细胞(ECV304)常压缺氧的保护作用.方法 以KM清洁级雄性小白鼠和ECV304为研究对象,通过小鼠密闭缺氧,记录刺五加高(25 ml/kg)低(12.5 ml/kg)剂量组小鼠因缺氧而死亡的时间；细胞培养,MTT法检测刺五加高(100 μL/mL)低(50 μL/mL)剂量组作用48 h后波长490 nm吸光度值.结果 腹腔注射刺五加注射液(12.5 mL/kg)、刺五加注射液(25 mL/kg)药物的小鼠,在密闭性缺氧条件下,其存活时间明显延长,与氯化钠组相比较有极显著差异,可明显提高小鼠对密闭性缺氧的耐受力.刺五加(12.5 mL/kg)组抗缺氧效果明显不如尼莫地平组,有极显著差异；刺五加(25 mL/kg)组与尼莫地平组抗缺氧效果相比较无明显差异；缺氧造成ECV304细胞存活率降低,与常氧氯化钠组相比,有极显著差异.
其他文献
Caffeine,arguably the most widely consumed psychoactive drugs,has been recently shown to protection neuronal injury in animal models of stroke,Parkinsons disease and multiple sclerosis.However,the mec
(1) Morroniside belongs to an extensive group of natural iridorid glycosides.In the present study,using human neuroblastoma SH-SY5Y cells,we have investigated the protective effects of this compound o
Stroke is a leading cause of death,and remains a major cause of disability.Up to now,few therapies,such as early t-PA application,are clinical effective along with narrow therapy time window and adver
Objective Tight junction (TJ) is an important structural and functional basis of maintaining blood-brain barrier (BBB) integrity.Bradykinin (BK) has been shown to selectively open blood-tumor barrier
Objective Voltage-gated sodium charmels play critical role in the electrical excitability of the brain.Of the known eleven sodium channel genes,four are broadly expressed throughout the central nervou
海马与学习、情绪、记忆等多种神经活动有关,应激能引起海马细胞受损,慢性应激选择性损害海马CA3区神经元,寻找海马细胞保护方法有重要意义。睾丸支持细胞可分泌多种生长因子,可能可以促进海马细胞的贴壁及生长。方法 新生SD乳鼠(出生48 h以内)的海马细胞分离培养,12-16天SD大鼠支持细胞分离培养,分为单纯DMEM组、支持细胞培养液组(SCM)、支持细胞直接共培养组,分别培养24 h、48 h、72
Purpose To investigate the mouse retinal structure during postnatal development with some antibodies specific for glial fibrillary acidic protein (GFAP),Vimentin which were considered as glial cells s
Purpose The electroporation of mouse retinal ganglion cells (RGCs) is not efficient after birth,as transfection of the differentiated neurons is much difficult than the newly born cells or the precurs
目的 观察当归提取物AS-A、AS-B、AS-C、AS-D、AS-E、AS-F、AS-G和AS-B-1对PC12细胞生长分化的影响和对PC12细胞缺氧损伤的保护作用.方法 实验分为空白组、NGF组和给药组.NGF组作为阳性对照,终浓度为20 ng/mL,给药各组在缺氧前24 h分别按25 μg/mL、50 μg/mL和100 μg/mL浓度加入当归不同提取物.将正常培养8天的细胞,移置恒温(37℃
目的 NB-3是1996年发现的神经黏附分子,属于免疫球蛋白超家族中Contactin/F3亚家族中的成员,在神经轴突导向和神经发育中具有重要作用.本实验检测了NB-3在小鼠大脑组织中不同时期的表达情况.方法 (1)主要采用现有的NB-3基因敲除的小鼠即胚胎18天(E18)的NB-3(+/-)胎鼠和8-10周的NB-3(+/-)成鼠的大脑组织,采用X-gal染色的方法检测LacZ基因产物β-半乳糖